Products
Online Inquiry
Our Products Cannot Be Used As Medicines Directly For Personal Use.
Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.
Native Bacillus stearothermophilus Phosphofructokinase
Cat No.
NATE-0551
Description
Phosphofructokinase is a kinase enzyme that phosphorylates fructose 6-phosphate in glycolysis. The enzyme-catalysed transfer of a phosphoryl group from ATP is an important reaction in a wide variety of biological processes. One enzyme that utilizes this reaction is phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, a key regulatory step in the glycolytic pathway. PFK exists as a homotetramer in bacteria and mammals (where each monomer possesses 2 similar domains) and as an octomer in yeast (where there are 4 alpha-(PFK1) and 4 beta-chains (PFK2), the latter, like the mammalian monomers, possessing 2 similar domains[3]). This protein may use the morpheein model of allosteric regulation.
Abbr
PFK, Native (Bacillus stearothermophilus)
Alias
PFKWII; PFK
Source
Bacillus stearothermophilus
Applications
Useful for enzymatic determiantion of fructose-6-phosphate
Appearance
White to pale yellow powder
Product Overview
Is an enzyme produced by microorganisms. This product shall be used for a diagnostics reagent.
Form
Freeze dried powder
Enzyme Commission Number
EC 2.7.1.11
Activity
> 250 U/mg
CAS No.
9001-80-3
Contaminants
NADPH oxidase < 0.01%; ATPase < 0.005%
Molecular Weight
72 kDa (gel filtration); 35 kDa (SDS‒PAGE)
Isoelectric point
pH 5.9
pH Stability
6.0-10.0 (37°C, 60 mins)
Michaelis Constant
D‒Fructose‒6‒phosphate (D‒F‒6‒P) 5.8 mM (at 37°C); ATP 0.07 mM (at 37°C)
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of fructose‒6‒phosphate to Fructose‒1,6 ‒bisphosphate per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
9
Activators
Mg2+
Thermal stability
Stable at 55°C and below (pH 8.5, 30 mins)
Storage
Storage at-20°C in the presence of a desiccant is recommended.
Synonyms
PFKWII; EC 2.7.1.11; PFK; phosphofructokinase; 6-phosphofructokinase; Phosphofructokinase I; Phosphohexokinase
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| DIA-403 | Native Phosphofructokinase from Thermophillic bacteria | EC 2.7.1.11 | 9001-80-3 | Thermophillic bacteria | Inquiry |