Description
The glucose oxidase enzyme (GOx) also known as notatin (EC number 1.1.3.4) is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present.
Abbr
GOD (Aspergillus sp.)
Applications
This enzyme is useful for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase in clinical analysis.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.3.4
Activity
GradeⅡ 100U/mg-solid or more (containing approx. 50% of stabilizers)
Contaminants
Catalase < 3.0%
Molecular Weight
approx. 153 kDa
pH Stability
pH 4.5-6.0 (30°C, 20hr)
Michaelis Constant
3.3×10⁻²M (ß-D-Glucose), 6.1×10⁻²M (2-Deoxyglucose)
Structure
Glycoprotein with 2 moles of FAD
Optimum temperature
40-50°C
Thermal stability
below 50°C (pH 5.7, 1hr)
Stability
Stable at-20°C for at least one year
Stabilizers
Potassium gluconate, sodium glutamate
Inhibitors
p-Chloromercuribenzoate, heavy metal ions (Cu⁺⁺, Hg⁺⁺, Ag⁺)
Synonyms
EC 1.1.3.4; glucose oxyhydrase; corylophyline; penatin; glucose aerodehydrogenase; microcid; β-D-glucose oxidase; D-glucose oxidase; D-glucose-1-oxidase; β-D-glucose:quinone oxidoreductase; glucose oxyhydrase; deoxin-1; GOD; 9001-37-0; glucose oxidase enzyme; GOx; notatin; glucose oxidase
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