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Hexokinase from Yeast, chemically modified
Cat No.
NATE-0989
Description
Chemically Modified Yeast Hexokinase for research on glucose metabolism and enzymatic activity. Perfect for biochemistry and molecular biology studies. Creative Enzymes ensures high-quality solutions.
Abbr
HK, chemically modified (Yeast)
Source
Yeast
Applications
Use Hexokinase in diagnostic tests for blood glucose using the hexokinase method and for the determination of creatine kinase.
Appearance
White lyophilizate
Product Overview
Recombinant enzyme that converts hexose to hexose-6-phosphate. Take advantage of the improved stability in liquid reagents. Rely on the proven diagnostic quality of this product.
Activity
> 40 U/mg lyophilizate
Contaminants
Alcohol dehydrogenase: <0.001 ATPase: <0.05 Creatine kinase: <0.001 G6P-DH: <0.005 Glutamate dehydrogenase: <0.05 Glutathione reductase: <0.005 Myokinase: <0.001 ''NADH oxidase'': <0.001 6-Phosphogluconate dehydrogenase: <0.001 Phophoglucose isomerase: <0.002 Phosphoglucomutase: <0.02 Glucose: <0.3 μg/mg lyophilizate
Molecular Weight
57 kD (SDS-PAGE)
Isoelectric point
4.5-5.0
pH Stability
5.0-9.0
Michaelis Constant
Phosphate buffer, 0.1 mol/l, pH 7.0; +25°C: 3.05 x 10-4 mol/l Phosphate buffer, 0.1 mol/l, pH 7.4; +30°C: 1.90 x 10-4 mol/l Tea buffer, 0.1 mol/l, pH 7.6; +25°C: 2.30 x 10-4 mol/l
Specificity
Hexokinase phosphorylates D-glucose, D-fructose, D-mannose, D-glucosamin, 2-deoxyglucose. L-Arabinose, D-xylose, L-rhamnose, D-galactose, D-lactose, sucrose, maltose, trehalose, raffinose, N-acetyl glucosamine do not react. ATP can be partially replaced by other nucleotides.
Optimum pH
7.0-10.0
Activators
Mg2+, catecholamines
Stability
At +2 to +8°C within specification range for 18 months. Store dry.
Inhibitors
EDTA, SH-blocking compounds, sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxymethylglucose, lyxose.
Synonyms
hexokinase type IV glucokinase; hexokinase D; hexokinase type IV; hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; hexokinase; ATP:D-hexose 6-phosphotransferase
Download
Download Datasheet"Hexokinase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1097 | Native Yeast Hexokinase | EC 2.7.1.1 | 9001-51-8 | Yeast | Inquiry |
| NATE-1156 | Native Rhodothermus obamensis Hexokinase | EC 2.7.1.1 | 9001-51-8 | Rhodothermus obamensis | Inquiry |
| NATE-1034 | Hexokinase from Kluyveromyces fragilis, Recombinant | EC 2.7.1.1 | 9001-51-8 | E. coli | Inquiry |
| NATE-0342 | Native Saccharomyces cerevisiae Hexokinase | EC 2.7.1.1 | 9001-51-8 | Saccharomyces cerevisiae | Inquiry |