Description
5'-nucleotidase is an extracellular enzyme that converts nucleoside-5'−monophosphates to nucleosides with a substrate preference of AMP. Native 5'-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5'-nucleotidase has many functions in vivo including the generation of extracellular adenosine. 5'-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research.1 It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.
Abbr
AMPase, Recombinant (Human)
Alias
AMPase; UMPase; CD73; NT5E
Form
Supplied as a solution containing Tris, NaCl, CaCl2, and 20% glycerol.
Enzyme Commission Number
EC 3.1.3.5
Molecular Weight
~61 kDa by SDS-PAGE (reducing)
Unit Definition
One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5'-monophosphate per min at pH 9.0 at 37 °C.
Synonyms
uridine 5'-nucleotidase; 5'-adenylic phosphatase; adenosine 5'-phosphatase; AMP phosphatase; adenosine monophosphatase; 5'-mononucleotidase; AMPase; UMPase; snake venom 5'-nucleotidase; thimidine monophosphate nucleotidase; 5'-AMPase; 5'-AMP nucleotidase; AMP phosphohydrolase; IMP 5'-nucleotidase; EC 3.1.3.5; CD73; NT5E; ecto-5'-nucleotidase
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