Description
The enzyme, characterized from the soil bacterium Bradyrhizobium sp. JS329, is involved in the pathway of 5-nitroanthranilate degradation. It is unusual in being able to catalyse the ring fission without the requirement for prior removal of the nitro group. The product undergoes spontaneous lactonization, with concurrent elimination of the nitro group.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
naaB (gene name); 5-nitrosalicylate:oxygen 1,2-oxidoreductase (decyclizing)
Reaction
5-nitrosalicylate + O2 = 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite (overall reaction); (1a) 5-nitrosalicylate + O2 = 4-nitro-6-oxohepta-2,4-dienedioate; (1b) 4-nitro-6-oxohepta-2,4-dienedioate = 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite (spontaneous)
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Download Datasheet