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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Cat No.
CSUB-0050
Applications
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Enzyme Commission Number
229-506-1
CAS No.
6578-06-9
Molecular Weight
433.62
Storage
2-8°C
Synonyms
BCIP p-toluidine salt; X-phosphate p-toluidine salt
Molecular Formula
C8H6BrClNO4P · C7H9N
MDL Number
MFCD00040636
PubChem Substance ID
24850179
Substrates
Alkaline Phosphatase
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