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5-Bromo-4-chloro-3-indolyl phosphate disodium salt
Cat No.
CSUB-0035
Applications
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
CAS No.
102185-33-1
Molecular Weight
370.43
Storage
-20°C
Synonyms
BCIP; X-phosphate disodium salt
MDL Number
MFCD00036757
Solubility
H2O: soluble 20 mg/mL
PubChem Substance ID
24891897
Substrates
Alkaline Phosphatase
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