Description
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. It is a non-fluorescent molecule that diffuses into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in cells with intact cell membranes. 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate does not stain dead cells but it can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells.
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