Description
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-chlorine bond, releasing a chloride and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme's activity can also result in β-replacement reactions, e.g. in the presence of hydrogen sulfide it can convert 3-chloro-D-alanine into D-cysteine and chloride.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
β-chloro-D-alanine dehydrochlorinase; 3-chloro-D-alanine chloride-lyase (deaminating)
Reaction
3-chloro-D-alanine + H2O = pyruvate + chloride + NH3 (overall reaction); (1a) 3-chloro-D-alanine = chloride + 2-aminoprop-2-enoate; (1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous); (1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Download Datasheet