Description
In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: androsterone + NAD (P)+ ↔ 5alpha-androstane-3,17-dione + NAD (P)H + H+. The 3 substRates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases.
Applications
Bile acid is one of the substrates of 3α-hydroxy steroiddehydrogenase. 3α-hydroxy steroid dehydrogenase is used to catalyzethe dehydrogenation reaction of hydroxy steroid in clinic. So, HSD is used to detect the total bile acid clinically.
Appearance
White powder, lyophilized
Enzyme Commission Number
EC 1.1.1.50
Activity
About 50U/mg powder
Molecular Weight
About 28 kDa (SDS-PAGE detection)
Purity
90% (SDS-PAGE test)
Unit Definition
One unit will catalyze the oxidation of 1µmol of androsterone per min at pH8.9 at 25°C.
Storage
4°C, store at -20°C for long-term preservation.
Synonyms
hydroxyprostaglandin dehydrogenase; 3α-hydroxysteroid oxidoreductase; sterognost 3α; 3α-hydroxysteroid dehydrogenase (B-specific); 3α-hydroxysteroid 3-dehydrogenase (B-specific); 3α-hydroxysteroid:NAD (P)+ 3-oxidoreductase (B-specific); EC 1.1.1.50
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