Description
3,3'-Diaminobenzidine tetrahydrochloride hydrate is a water-soluble preparation of 3,3'-Diaminobenzidine, a tetraamine biphenyl compound used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
Applications
A widely used chromogenic peroxidase substrate.
Storage
Desiccate at room temperature
Synonyms
3,3',4,4'-Tetraaminobiphenyl tetrahydrochloride
Molecular Formula
C12H14N4•4HCl
Solubility
Soluble in water.
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