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3,3',5,5'-Tetramethylbenzidine
Cat No.
CSUB-0445
Applications
3,3',5,5'-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
Enzyme Commission Number
259-364-6
CAS No.
54827-17-7
Molecular Weight
240.34
Storage
2-8°C
Synonyms
BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; TMB
MDL Number
MFCD00007748
PubChem Substance ID
24900090
mp
168-171 °C(lit.)
Beilstein Registry Number
2808541
Substrates
Peroxidase Substrates
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