Applications
2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Enzyme Commission Number
250-396-6
Synonyms
AzBTS-(NH4)2, Diammonium 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
Molecular Formula
C18H24N6O6S4
PubChem Substance ID
24891410
Reconstitution
Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide
Beilstein Registry Number
7329461
Substrates
Peroxidase Substrates
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