Description
Enzymatically converts β-D-glucose-1-phosphate to β-D-glucose-6-phosphate. β-Phosphoglucomutase enzymatically converts β-D-glucose 1-phosphate to β-D-glucose 6-phosphate. It is involved in starch and sucrose metabolism. This enzyme belongs to the family of isomerases, specifically the phosphotransferases (phosphomutases), which transfer phosphate groups within a molecule. This enzyme participates in starch and sucrose metabolism.
Abbr
β-Phosphoglucomutase, Recombinant (E. coli)
Appearance
White lyophilizate
Enzyme Commission Number
EC 5.4.2.6
Activity
> 20 U/mg lyophilizate
Contaminants
α-amylase < 5.0 x 10^-3% α-glucosidase < 5.0 x 10^-2% NADPH oxidase < 5.0 x 10^-2%
Molecular Weight
ca. 34 kDa
Michaelis Constant
2.3 x 10^-4 M (β-D-glucose-1-phosphate)
Structure
monomer of ca. 25 kDa (SDS-PAGE)
Unit Definition
One unit (U) is defined as the amount of enzyme which converts 1 μmol of β-D-glucose-1-phosphate to β-D-glucose-6-phosphate per min at 37°C and pH 7.0.
Activators
Mg2+, Mn2+, Co2+, Ni2+
Thermal stability
below 45°C
Stability
Stability (liquid form) stable at 37°C for at least one week Stability (powder form) stable at 30°C for at lest one month
Stabilizers
Lactose, EDTA
Inhibitors
Hg2+, Zn2+, Cu2+, Cd2+
Synonyms
β-phosphoglucomutase; β-D-glucose 1,6-phosphomutase; EC 5.4.2.6
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