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β-Mannosidase from Cellvibrio mixtus, Recombinant

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Cat No.

NATE-1188

Description

Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.

Abbr

β-Mannosidase, Recombinant (Cellvibrio mixtus)

Source

Cellvibrio mixtus

Form

Supplied in 3.2 M ammonium sulphate

Enzyme Commission Number

EC 3.2.1.25

Activity

40 U/mg

CAS No.

9025-43-8

Molecular Weight

50300 Da

Purity

> 95 % as judged by SDS-PAGE

Concentration

120 U/ml

Unit Definition

One unit is defined as the amount of enzyme required to release 1μmol of p-nitrophenol per hour from p-nitrophenyl-β-mannopyranoside (1 mM in the assay) in 50 mM phosphate buffer, pH 7.0, at 37°C, containing 1 mg/ml of BSA.

Optimum pH

7 (stable from 6.5 – 7.5)

Optimum temperature

37°C (stable up to 40°C)

Storage

Store at 4°C (shipped at room temperature)

Synonyms

β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8

"β-Mannosidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0778	Native Helix pomatia β-Mannosidase	EC 3.2.1.25	9025-43-8	Helix pomatia	Inquiry
NATE-1185	β-Mannosidase from Bacteroides fragilis, Recombinant	EC 3.2.1.25	9025-43-8	Bacteroides fragilis NCTC 9343	Inquiry
NATE-1186	β-Mannosidase from Bacteroides thetaiotaomicron, Recombinant	EC 3.2.1.25	9025-43-8	Bacteroides thetaiotaomicron VPI-5482	Inquiry
NATE-1187	β-Mannosidase from Cellvibrio japonicus, Recombinant	EC 3.2.1.25	9025-43-8	Cellvibrio japonicus NCIMB 10462	Inquiry

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