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β-Mannosidase 1A from Pyrococcus furiosus, Recombinant

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Cat No.

NATE-1470

Description

Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.

Abbr

β-Mannosidase 1A, Recombinant (Pyrococcus furiosus)

Source

E. coli

Species

Pyrococcus furiosus

Form

35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol

Enzyme Commission Number

EC 3.2.1.25

CAS No.

37288-54-3

Molecular Weight

61.1 kDa

Purity

>90% by SDS-PAGE

Concentration

0.25 mg/mL

Specificity

p-nitrophenyl-β-D-mannopyranoside (ManpbNp), p-nitrophenyl-α-glucopyranoside (GlcpbNp)

Optimum pH

Optimum temperature

95 °C

Storage

This enzyme is shipped at room temperature but should be stored at -20 °C.

Synonyms

β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8

"β-Mannosidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0778	Native Helix pomatia β-Mannosidase	EC 3.2.1.25	9025-43-8	Helix pomatia	Inquiry
NATE-1185	β-Mannosidase from Bacteroides fragilis, Recombinant	EC 3.2.1.25	9025-43-8	Bacteroides fragilis NCTC 9343	Inquiry
NATE-1186	β-Mannosidase from Bacteroides thetaiotaomicron, Recombinant	EC 3.2.1.25	9025-43-8	Bacteroides thetaiotaomicron VPI-5482	Inquiry
NATE-1187	β-Mannosidase from Cellvibrio japonicus, Recombinant	EC 3.2.1.25	9025-43-8	Cellvibrio japonicus NCIMB 10462	Inquiry

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