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α-Lytic Protease M190A Mutant, Recombinant

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Cat No.

NATE-0051

Description

Alpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications, whose wild-type (WT) version cleaves after T, A, S, and V residues. The M190A (Met190 → Ala190) mutant of aLP has different cleavage specificities, and cleaves after M, F, and L residues. Both the WT and M190A forms of aLP geneRate peptides of similar average length as trypsin.

Abbr

ALP M190A, Recombinant

Alias

ALP M190A

Activity

> 0.05 U/mg

Isoelectric point

9.69

Unit Definition

mmols of pNA produced per min per mg protein from 0.5 mM N-succinyl-Ala-Ala-Pro-Phe-PNA at 25°C in 100 mM Tris-HCl (pH 7.5).

Optimum pH

5.0(storage); 7.5(activity)

Storage

−70°C

Synonyms

ALP M190A; Alpha-Lytic Protease M190A Mutant

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