Description
Recombinant Homocysteine α,γ-lyase is a pyridoxal-5’-phosphate dependent enzyme. It is a mutant of homocysteinase from Trichomonas vaginalis encoded by mgl1 gene, containing three point mutations, such as; Phe47Leu, Asp172Glu, Ser308Tyr. The enzyme can metabolize homocysteine into α-keto butyrate, hydrogen sulfide and ammonia.
Abbr
rHCYase, Recombinant (T. vaginalis)
Source
E. coli and fused to His-tag at N-terminus
Species
Trichomonas vaginalis
Unit Definition
One Unit enzyme converts 1 µmole of homocysteine into hydrogen sulfide, per minute at 25°C and pH 8.1 in the presence of pyridoxal phosphate.
Storage
Reconstituted enzyme can be stored in working aliquots at -20°C and use within 3 months. Avoid repeated freeze-thaw cycles.
Synonyms
α,γ-Homocysteinase; Methionine gamma-lyase; mgl1
Reconstitution
Reconstitute in 40 mM Sodium phosphate buffer, pH 8.1.
Formulation
In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol
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