Description
Kappa-carrageenase (EC 3.2.1.83, kappa-carrageenan 4-beta-D-glycanohydrolase) is an enzyme with systematic name kappa-carrageenan 4-beta-D-glycanohydrolase (configuration-retaining). This enzyme catalyses the following chemical reaction: Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans. The main products of hydrolysis are neocarrabiose-sulfate and neocarratetraose-sulfate.
Abbr
K-Carrageenase, Recombinant (Zobellia galactanivorans)
Species
Zobellia galactanivorans
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.83
Molecular Weight
34.2 kDa
Specificity
K-carrageenan
Optimum temperature
20-30 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
K-Carrageenase; Kappa-carrageenase; EC 3.2.1.83; kappa-carrageenan 4-beta-D-glycanohydrolase
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