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β-Agarase I from E. coli, Recombinant
Cat No.
NATE-1282
Description
Recombinant Agarase I from E. coli for research on agar hydrolysis and enzymatic mechanisms. Perfect for microbiology and molecular biology studies. Creative Enzymes delivers trusted products.
Abbr
β-Agarase I, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Applications
β-Agarase I digests agarose, releasing trapped DNA and producing carbohydrate molecules which can no longer gel. β-Agarase I can be used to purify both large (> 50 kb) and small (< 50 kb) fragments of DNA from gels. The remaining carbohydrate molecules and β-Agarase I will not, in general, interfere with subsequent DNA manipulations such as restriction endonuclease digestion, ligation, and transformation.
Product Overview
β-Agarase cleaves the agarose subunit, unsubstituted neoagarobiose [3,6-anhydro-a-L-galactopyranosyl-1-3-d-galactose] to neoagaro-oligosaccharides.
Form
50 mM Bis Tris-HCl (pH 6.5), 1 mM Na2EDTA and 50% glycerol.
Concentration
1,000 units/ml
Unit Definition
One unit is defined as the amount of enzyme required to digest 200 μl of molten low melting point or NuSieve agarose to nonprecipitable neoagaro-oligosaccharides in 1 hour at 42°C.
Storage
at -20°C
Synonyms
agarase; AgaA; AgaB; endo-β-agarase; agarose 3-glycanohydrolase; EC 3.2.1.81; 37288-57-6
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0040 | Native Pseudomonas atlantica Agarase | EC 3.2.1.81 | 37288-57-6 | Pseudomonas atlantica | Inquiry |
| NATE-1293 | β-Agarase 16D from Zobellia galactanivorans, Recombinant | EC 3.2.1.81 | 37288-57-6 | E. coli | Inquiry |
| EXWM-3945 | β-agarase | EC 3.2.1.81 | 37288-57-6 | Inquiry |