Description
Catalysis of the hydrolysis of the glycosidic bond in an unsaturated saccharide between the unsaturated glucuronyl residue at the nonreducing terminus and the saccharide linked to the residue.
Abbr
Δ-4,5-unsaturated β-glucuronyl hydrolase, Recombinant (Pedobacter heparinus)
Species
Pedobacter heparinus
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.-
Molecular Weight
45.7 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
Heparin/heparan sulfate
Optimum temperature
30 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
d-4,5 unsaturated beta-glucuronyl hydrolase; EC 3.2.1.-; unsaturated beta-glucuronyl hydrolase
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