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α-2,3-Sialyltransferase from Pasteurella multocida, Recombinant
Cat No.
NATE-1171
Description
Sialyltransferases are enzymes that transfer sialic acid to nascent oligosaccharide. Each sialyltransferase is specific for a particular sugar substrate. Sialyltransferases add sialic acid to the terminal portions of the sialylated glycolipids (gangliosides) or to the N-or O-linked sugar chains of glycoproteins. Sialyltransferases belong to glycosyltransferase family 29 (CAZY GT_29) which use a nucleotide monophosphosugar as the donor (CMP-NeuA) instead of a nucleotide diphosphosugar.
Abbr
α-2,3-Sialyltransferase, Recombinant (Photobacterium damsela)
Source
E. coli BL21
Species
Photobacterium damsela
Form
Lyophilized powder containing Tris-HCl and NaCl.
Enzyme Commission Number
EC 2.4.99.4
Activity
> 2 units/mg
CAS No.
71124-51-1
Molecular Weight
46.4 kDa
Isoelectric point
5.94
Unit Definition
One unit will catalyze the formation of 1.0 mmole of Neu-5-Ac-a-2,3-Lac-MU from CMP-Neu-5-Ac and Lac-b-O-MU per minute at 37 °C at pH 8.0.
Optimum pH
7.5–8.5
Storage
Store the product at –20 °C. It remains active for at least 1 year when stored properly.
Preparation Instructions
Reconstitute the lyophilized powder with water to ~5 mg/mL. Solutions can be stored at 2–8 °C for 1–2 months after reconstitution. They can also be aliquoted and frozen at –70 °C or –20 °C for 1 year. Multiple freeze-thaw cycles should be avoided.
Synonyms
α (2,3)-Sialyltransferase; Beta-galactoside alpha-2,3-sialyltransferase; Beta-galactosamide alpha-2,3-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase
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Download Datasheet"α-Sialyltransferase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1921 | α-2,3/2,6-Sialyltransferase from Pasteurella multocida, recombinant | EC 2.4.99.1, 2.4.99.4, 3.2.1.- | Pasteurella multocida | Inquiry | |
| NATE-0759 | α(2,6)-Sialyltransferase from Photobacterium damsela, Recombinant | EC 2.4.99.1 | 9075-81-4 | E. coli BL21 | Inquiry |
| NATE-0988 | α-2,3-Sialyltransferase, Recombinant | Inquiry | |||
| NATE-1476 | α-2,3/8-sialyltransferase from Campylobacter jejuni, Recombinant | EC 2.4.99.- | E. coli | Inquiry |