Description
Digestion of human IgG1 with Unique protease 1 generates a homogenous pool of precise Fab and Fc fragments. There is no over-digestion or further degradation of the fragments typically associated with other proteolytic enzymes. Very mild reducing reaction conditions give intact Fab and Fc fragments. Best activity is obtained at 37°C.
Abbr
Unique protease 1 (Porphyromonas gingivalis)
Source
Porphyromonas gingivalis
Appearance
White to light yellow powder
Form
Lyophilized preparation together with 5 vials of lyophilized reducing agent for convenient and reliable performance.
Purity
> 80% homogeneity as determined by SDS-PAGE analysis using Coomassie Blue detection.
Unit Definition
One unit cleaves > 95% of 1μg human IgG1 when incubated in 0.1 M Tris, pH 8.0 at 37°C for 1 hour.
Stability
After reconstitution Unique protease 1 is stable for at least 1 month +4-8°C. Reconstituted Unique protease 1 Reducing Agent cannot be stored, use freshly prepared.
Storage
Unique protease 1 and Unique protease 1 Reducing Agent are shipped on ice and should be stored at -20°C upon arrival.
Synonyms
GingisKHAN; unique protease 1
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