Description
In eukarya an additional guanosine residue is added post-transcriptionally to the 5'-end of tRNAHis molecules. The addition occurs opposite a universally conserved adenosine73 and is thus the result of a non-templated 3'-5' addition reaction. The additional guanosine residue is an important determinant for aminoacylation by EC 6.1.1.21, histidyl-tRNA ligase.The enzyme requires a divalent cation for activity. ATP activation is not required when the substrate contains a 5'-triphosphate (ppp-tRNAHis).
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
histidine tRNA guanylyltransferase; Thg1p (ambiguous); Thg1 (ambiguous)
Reaction
p-tRNAHis + ATP + GTP = pppGp-tRNAHis + AMP + diphosphate (overall reaction); (1a) p-tRNAHis + ATP = App-tRNAHis + diphosphate; (1b) App-tRNAHis + GTP = pppGp-tRNAHis + AMP
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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