Description
Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig. Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins.
Abbr
TGC, Recombinant (Human)
Alias
TGase C; TGC; TG(C); TG2; TGM2
Applications
Western-Blot
Product Overview
Tissue Transglutaminase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 78,018 Dalton. tTG is expressed with a -6x His tag and purified by proprietary chromatographic techniques. By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating.
Enzyme Commission Number
EC 2.3.2.13
Molecular Weight
78,018 Da
Purity
Greater than 95% as determined by SDS-PAGE.
Concentration
0.6-1.4 µg/ml
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°Cfor longer periods of time. Avoid multiple freeze-thaw cycles.
Buffer
TGM2 is supplied in 16mM HEPES buffer pH-8.0, 320mM NaCl, and 20% glycerol.
Function
1. Binds IgA & IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
Synonyms
Protein-glutamine gamma-glutamyltransferase 2; EC 2.3.2.13; Tissue transglutaminase; TGase C; TGC; TG(C); Transglutaminase-2; TGase-H; TG2; TGM2
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