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T4 deoxyribonuclease II
Cat No.
EXWM-3578
Description
Requires Mg2+. This phage T4 enzyme is involved in degradation of host DNA. The enzyme primarily catalyses nicking of the bottom strand of double stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Double-stranded breaks are produced 5- to 10-fold less frequently. It does not cleave the T4 native DNA, which contains 5-hydroxymethylcytosine instead of cytosine.
Form
Liquid or lyophilized powder
Enzyme Commission Number
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
T4 endonuclease II; EndoII (ambiguous); denA (gene name)
Reaction
Endonucleolytic nicking and cleavage of cytosine-containing double-stranded DNA.
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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