Description
Polyphosphate Kinase catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.
Abbr
Polyphosphate Kinase, Recombinant (Propionibacterium shermanii)
Source
Propionibacterium shermanii
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Enzyme Commission Number
EC 2.7.4.1
Unit Definition
The amount of Polyphosphate kinase required to convert 1 µmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.
Stability
Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Buffer
The protein was lyophilized from 1.15ml PPK solution containing 43.6 U/ml of PPK activity, 0.77 mg/ml total protein, 10mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.
Synonyms
Polyphosphate kinase; EC 2.7.4.1; Polyphosphoric acid kinase; ATP-polyphosphate phosphotransferase
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