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Pectate Lyase from Clostridium thermocellum, Recombinant
Cat No.
NATE-1556
Description
Pectate lyase (EC 4.2.2.2) is an enzyme involved in the maceration and soft rotting of plant tissue. Pectate lyase is responsible for the eliminative cleavage of pectate, yielding oligosaccharides with 4-deoxy-α-D-mann-4-enuronosyl groups at their non-reducing ends. The protein is maximally expressed late in pollen development. It has been suggested that the pollen expression of pectate lyase genes might relate to a requirement for pectin degradation during pollen tube growth. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides.
Abbr
Pectate Lyase, Recombinant (Clostridium thermocellum)
Source
E. coli
Species
Clostridium thermocellum
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 4.2.2.2
CAS No.
9015-75-2
Molecular Weight
33.8 kDa
Purity
>90% as judged by SDS-PAGE
Concentration
1 mg/mL
Specificity
Polygalacturonic acid (PGA) and pectin (up to 55% methyl-esterified) from citrus fruits
Optimum pH
7.0-10.4
Optimum temperature
55 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
(1->4)-alpha-D-galacturonan lyase; polygalacturonic transeliminase; pectic acid transeliminase; polygalacturonate lyase; endopectin methyltranseliminase; pectate transeliminase; endogalacturonate transeliminase; pectic acid lyase; pectic lyase; alpha-1,4-D-endopolygalacturonic acid lyase; PGA lyase; PPase-N; endo-alpha-1,4-polygalacturonic acid lyase; polygalacturonic acid lyase; pectin trans-eliminase; Polygalacturonic acid trans-eliminase; Pectate lyase; EC 4.2.2.2
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Download Datasheet"Pectate Lyase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0908 | Pectate Lyase from Aspergillus sp., Recombinant | EC 4.2.2.2 | 9015-75-2 | Aspergillus sp. | Inquiry |
| NATE-0909 | Pectate Lyase from Cellvibrio japonicus, Recombinant | EC 4.2.2.2 | 9015-75-2 | Cellvibrio japonicus | Inquiry |
| NATE-1558 | Pectate Lyase from Erwinia chrysanthemi, Recombinant | EC 4.2.2.2 | 9015-75-2 | E. coli | Inquiry |
| NATE-1554 | Pectate Lyase from Bacteroides thetaiotaomicron, Recombinant | EC 4.2.2.2 | 9015-75-2 | E. coli | Inquiry |