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Pectate Lyase from Clostridium thermocellum, Recombinant

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Cat No.

NATE-1556

Description

Pectate lyase (EC 4.2.2.2) is an enzyme involved in the maceration and soft rotting of plant tissue. Pectate lyase is responsible for the eliminative cleavage of pectate, yielding oligosaccharides with 4-deoxy-α-D-mann-4-enuronosyl groups at their non-reducing ends. The protein is maximally expressed late in pollen development. It has been suggested that the pollen expression of pectate lyase genes might relate to a requirement for pectin degradation during pollen tube growth. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides.

Abbr

Pectate Lyase, Recombinant (Clostridium thermocellum)

Source

E. coli

Species

Clostridium thermocellum

Form

35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol

Enzyme Commission Number

EC 4.2.2.2

CAS No.

9015-75-2

Molecular Weight

33.8 kDa

Purity

>90% as judged by SDS-PAGE

Concentration

1 mg/mL

Specificity

Polygalacturonic acid (PGA) and pectin (up to 55% methyl-esterified) from citrus fruits

Optimum pH

7.0-10.4

Optimum temperature

55 °C

Storage

This enzyme is shipped at room temperature but should be stored at -20 °C.

Synonyms

(1->4)-alpha-D-galacturonan lyase; polygalacturonic transeliminase; pectic acid transeliminase; polygalacturonate lyase; endopectin methyltranseliminase; pectate transeliminase; endogalacturonate transeliminase; pectic acid lyase; pectic lyase; alpha-1,4-D-endopolygalacturonic acid lyase; PGA lyase; PPase-N; endo-alpha-1,4-polygalacturonic acid lyase; polygalacturonic acid lyase; pectin trans-eliminase; Polygalacturonic acid trans-eliminase; Pectate lyase; EC 4.2.2.2

"Pectate Lyase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0909	Pectate Lyase from Cellvibrio japonicus, Recombinant	EC 4.2.2.2	9015-75-2	Cellvibrio japonicus	Inquiry
NATE-0908	Pectate Lyase from Aspergillus sp., Recombinant	EC 4.2.2.2	9015-75-2	Aspergillus sp.	Inquiry
NATE-1222	Pectate Lyase from Caulobacter crescentus, Recombinant	EC 4.2.2.2	9015-75-2	Caulobacter crescentus CB15	Inquiry
NATE-1223	Pectate Lyase from Clostridium acetobutylicum, Recombinant	EC 4.2.2.2	9015-75-2	Clostridium acetobutylicum ATCC 824	Inquiry

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