Description
Presence of cytochrome oxidase can be detected through the use of an oxidase reagent, which acts as an electron donor to cytochrome oxidase. If the bacteria oxidize the reagent, it turns dark purple due to the formation of indophenol blue indicating a positive reaction. This test is fit for screening species of Neisseria, Aeromonas, Vibrio, Campylobacter and Pseudomonas, which show a positive reaction, or for Enterobacteriaceae, which show a negative reaction.