Description
In enzymology, an oxalate oxidase (EC 1.2.3.4) is an enzyme that catalyzes the chemical reaction: oxalate + O2 + 2 H+ ↔ 2 CO2 + H2O2.
The 3 substrates of this enzyme are oxalate, O2, and H+, whereas its two products are CO2 and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. The systematic name of this enzyme class is oxalate:oxygen oxidoreductase. This enzyme participates in glyoxylate and dicarboxylate metabolism. It has 2 cofactors: FAD, and Manganese.
Abbr
OxOx, Recombinant (B. subtilis)
Enzyme Commission Number
EC 1.2.3.4
Molecular Weight
43.6 kDa, His-tagged
Unit Definition
Defined as the amount of enzyme that catayze the conversion of converts 1 µmole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25°C.
Stability
Stable for at least 2 years as supplied.
Synonyms
OxO; OxOx; OxO_r; aero-oxalo dehydrogenase; oxalic acid oxidase
Reconstitution
Reconstitute to 2 mg/mL in sterile water, store at -80°C in aliquots and use within 6 months after reconstitution. Avoid repeated
freeze-thaw cycles.
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