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Oxalate Decarboxylase from Bacillus subtilis, recombinant
Cat No.
NATE-1688
Description
Oxalate decarboxylase (OxdC, EC4.1.1.2) is a manganese-containing enzyme, which decomposes oxalic acid and oxalate. With OxdC catalysis, oxalate is split into formate and CO2. This enzyme belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is oxalate carboxy-lyase (formate-forming). This enzyme is also called oxalate carboxy-lyase. The enzyme is composed of two cupin domains, each of which contains a Mn (II) ion. This enzyme participates in glyoxylate and dicarboxylate metabolism. This enzyme has been recognized for diagnostics in diverse biotechnological applications such as the clinical assay of oxalate in blood and urine, therapeutics, process industry, and agriculture to lower oxalate levels in foods and the environment. The recombinant protein made from the Bacillus Subtilis sequence includes OxdC with N-terminal His-tag.
Abbr
OxdC, Recombinant (Bacillus subtilis)
Source
E. coli
Species
Bacillus subtilis
Form
Liquid
Enzyme Commission Number
EC 4.1.1.2
Activity
150U/mg
CAS No.
9024-97-9
Molecular Weight
45.9 kDa
Purity
> 98% by SDS-PAGE
Concentration
2 mg/mL
Unit Definition
One unit is the amount of enzyme that generates 1.0 µmole of NADH at 37°C. Specific activity was expressed as U/mg protein.
Storage
Stable for 1 year at -20°C. For long term storage, aliquot and store at -70°C. Avoid repeated freezing and thawing cycles.
Synonyms
Oxalate carboxy-lyase; EC 4.1.1.2; Oxalate decarboxylase; OxdC
Formulation
In 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.
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