Description
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical. Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.
Abbr
Nitroreductase, Recombinant (E. coli)
Applications
Nitroreductase has been used in a study that used a set of PCR primers to clone a gene encoding a hypothetical nitroreductases (named as Ssap-NtrB) from uropathogenic staphyl oc occus. It has also been used to improve prodrug activation. Nitroreductase from Escherichia coli has been used in a study to assess anaerobic bacteria as a gene delivery system for cancer treatment. It has also been used in a study to investigate its applications in antibody-directed enzyme prodrug therapy.
Molecular Weight
monomer mol wt 24 kDa
Unit Definition
One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37°C.
Warnings
Produced using animal component-free materials.
Pathway
Microbial metabolism in diverse environments, organism-specific biosystem; Nitrotoluene degradation, organism-specific biosystem; Nitrotoluene degradation, conserved biosystem
Download Datasheet