Description
Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.
Abbr
FBP, Native (Propionibacterium freudenreichii (shermanii))
Source
Propionibacterium freudenreichii (shermanii)
Applications
FBP was used to study the kinetic mechanism of pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii.
Form
lyophilized powder; Contains imidazole salts and stabilizer
Enzyme Commission Number
EC 2.7.1.90
Activity
4.0-8.0 units/mg protein
Unit Definition
One unit will convert 1.0 μmole of pyrophosphate and fructose 6-phosphate to fructose 1,6-diphosphate and inorganic phosphate per min at pH 7.4 at 30°C.
Warnings
These two pyrophosphate-dependent fructose-6-phosphate kinases differ in their response to the physiological activator fructose 2,6-diphosphate [Fru (2,6)-P2]. The enzyme from Propionibacterium freudenreichii (F8381) reportedly is not affected by Fru (2,6)-P2. The enzyme from potato tubers (F2258) is stimulated by Fru (2,6)-P2 (half-maximal activation at 5.5 nM Fru (2,6)-P2 for potato tuber enzyme).
Synonyms
EC 2.7.1.90; 6-phosphofructokinase (pyrophosphate); pyrophosphate-fructose 6-phosphate 1-phosphotransferase; inorganic pyrophosphate-dependent phosphofructokinase; inorganic pyrophosphate-phosphofructokinase; pyrophosphate-dependent phosphofructo-1-kinase; pyrophosphate-fructose 6-phosphate phosphotransferase; 55326-40-4
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