Description
Citrate synthase catalyses the conversion of Citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Alias
CS; Citrate Synthase
Source
Pigeon breast muscle
Form
ammonium sulfate suspension. Crystalline suspension in 2.2 M (NH4)2SO4 solution, pH 7.0, containing 6 mM phosphate and 0.5 mM Citrate
Enzyme Commission Number
EC 4.1.3.7
Activity
80-150 units/mg protein (modified Warburg-Christian)
Unit Definition
One unit will form 1.0 μmole of Citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37°C.
Pathway
2-Oxocarboxylic acid metabolism, organism-specific biosystem; Carbon metabolism, organism-specific biosystem; Citrate cycle (TCA cycle, Krebs cycle), organism-specific biosystem
Synonyms
CS; EC 4.1.3.7; EC 2.3.3.1; 9027-96-7; Citrate (Si)-synthase; (R)-citric synthase; Citrate oxaloacetate-lyase [(pro-3S)-CH2COO-->acetyl-CoA]
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