Description
Topoisomerase II α (TopoIIα) is a gene product with conserved catalytic activities and it promotes the progression of DNA damage. The α isoform is present in proliferating cells.
Abbr
TOPO II, Native (Human)
Alias
TOPO II; DNA gyrase
Applications
Topoisomerase II α has been used in a study to assess aging pr ocesses in the human brain. Topoisomerase II α has also been used in a study to investigate its activity in HIV-1 replication.
Form
liquid; Solution in 10 mM Tris-HCl, pH 7.1, 0.25 M NaCl, 1 mM EDTA, 0.5 mM PMSF, 1 mM 2-mercaptoethanol, 10% glycerol.
Enzyme Commission Number
EC 5.99.1.3
Molecular Weight
mol wt 170 kDa
Unit Definition
One unit will decatenate 0.2 μg of kinetoplast DNA in 30 minutes at 37°C.
Warnings
Recombinant enzyme overexpressed in a proprietary eukaryotic cell line.
Pathway
Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; G0 and Early G1, organism-specific biosystem; Mitotic G1-G1/S phases, organism-specific biosystem
Function
ATP binding; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA-dependent ATPase activity; chromatin binding; drug binding; enzyme binding; histone deacetylase binding; nucleotide binding; protein C-terminus binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase C binding; sequence-specific DNA binding; structure-specific DNA binding; ubiquitin binding
Synonyms
type II DNA topoisomerase; DNA-gyrase; deoxyribonucleate topoisomerase; deoxyribonucleic topoisomerase; topoisomerase; DNA topoisomerase II; DNA topoisomerase (ATP-hydrolysing); EC 5.99.1.3; Topoisomerase II α; TOPO II
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