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Native Fig tree latex Ficin

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Cat No.

NATE-0255

Description

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab')2 fragments from mouse IgG1.

Abbr

Ficin, Native (Fig tree latex)

Alias

ficin; debricin

Source

Fig tree latex

Form

saline suspension or lyophilized powder

Enzyme Commission Number

EC 3.4.22.3

Activity

> 1.0 units/mg protein

CAS No.

9001-33-6

Molecular Weight

23.8 kDa

Specificity

Extinction Coefficient: E1% = 21.0 (280 nm) pI: 9.0

Unit Definition

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).

Synonyms

ficin; debricin; higueroxyl delabarre; EC 3.4.22.3; 9001-33-6; ficain

"Ficin" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1865	Immobilized Ficin (Agarose Resin)	EC 3.4.22.3			Inquiry
EXWM-4204	ficain	EC 3.4.22.3	9001-33-6		Inquiry