Description
A thermostable β-1,6-endoglucanase or pustulanase (E.C. 3.2.1.75) which catalyses the cleavage of β-1,6 bonds in pustulan and other beta-glucans containing 1,6 linked glucose units. The enzyme is now a component in a standard assay for beta-glucan from Baker’s yeast as documented in US Pharmacopeia Food and Chemicals Index.
Abbr
Pustulanase, Native (Environmental DNA)
Source
Proprietary metagenome environmental DNA
Species
Environmental DNA
Enzyme Commission Number
EC 3.2.1.75
Specificity
Cel136 Beta-glucanase (Pustulanase) Cel136 hydrolyses β-glucan polysaccharides with β-(1,6) linkages such as Pustulan (β-(1,6)-D-glucan) and Gentiobiose (β-(1,6)-linked disaccharide) or β-glucans with mixed linkages, including β-(1,6) linkages, such as Laminarin (β-(1,3/1,6)-D-glucan) and Yeast β-glucan (β-(1,3/1,6)-D-glucan). Substrate specificity analysis (below) indicates that the enzyme does not cleave β-(1,3) or β-(1,4) linkages as it does not show activity on the β-glucans Lichenan (β-(1,3/1,4)-D-glucan) or CM-Cellulose (β-(1,4)-D-glucan).
Unit Definition
One unit (U) of enzyme activity is the amount that leads to the release of 1 μmol reducing sugars per minute.
Optimum pH
suitable pH range is about 5-8 with optimum around 5.5
Optimum temperature
The enzyme is relatively active in a rather broad temperature range (65-90°C)with optimum around 80°C
Synonyms
EC 3.2.1.75; glycoside hydrolase; pustulanase; glucan endo-1,6-β-glucosidase; 6-β-D-glucan glucanohydrolase; endo-1,6-β-glucanase; β-1→6)-β-D-glucanase; β-1,6-glucanase-pustulanase; β-1,6-glucan hydrolase; β-1,6-glucan 6-glucanohydrolase; 1,6-β-D-glucan glucanohydrolase
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