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Native Chicken Egg White Avidin
Cat No.
NATE-0949
Description
Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity. Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including Western Blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support. Avidin and biotin are routinely conjugated to indicator enzymes, such as alkaline phosphatase and horseradish peroxidase for use in diagnostic kit development and manufacturing.
Abbr
Avidin, Native (Chicken Egg White)
Source
Chicken Egg White
Species
Chicken
Applications
Egg white Avidin can be used in assays for functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Form
Lyophilized
Enzyme Commission Number
215-783-6
Activity
> 10 U/mg solid
CAS No.
1405-69-2
Contaminants
Ash: < 0.1%
Molecular Weight
64,000 (tetramer of 16,000 MW subunits)
Purity
Highly Purified
Unit Definition
One unit will bind one microgram of d-biotin.
Stability
3 years
Storage
2-8°C
Synonyms
Avidin
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