Description
In enzymology, phenylpyruvate tautomerase or Macrophage migration inhibitory factor (EC 5.3.2.1) is an enzyme that catalyzes the chemical reaction:keto-phenylpyruvate↔ enol-phenylpyruvate. Phenylpyruvate tautomerase has also been found to exhibit the same keto-enol tautomerism for 4-Hydroxyphenylpyruvic acid, which is structurally similar to phenylpyruvate but contains an additional hydroxyl moiety in the para position of the aromatic ring. This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting keto-and enol-groups. This enzyme participates in tyrosine metabolism and phenylalanine metabolism.
Abbr
Tautomerase, Native (Bovine)
Applications
Tautomerase from bovine kidney has been used in a study to assess tritium isotope effects in the reaction catalyzed by 4-hydroxyphenylpyruvate dioxygenase. Tautomerase from bovine kidney has also been used in a study to investigate human macrophage migration inhibitory factor.
Enzyme Commission Number
EC 5.3.2.1
Activity
1-4 units/mg protein (Lowry), ~10 units/mL
Concentration
~10 units/mL
Unit Definition
One unit will produce a first-order rate constant (k) of 1.0 at pH 6.2 at 25°C, using p-hydroxyphenylpyruvate (keto). Reaction vol = 3.3 ml.
Synonyms
Tautomerase; phenylpyruvate tautomerase; EC 5.3.2.1; phenylpyruvic keto-enol isomerase; 9023-54-5
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