Description
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. In vitro, its optimum pH range is 4.5-5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6-5.9. The molecular weight is approximately 38 kDa Da.
Abbr
DNASE2, Native (Bovine)
Alias
DNase II alpha; DNASE2
Applications
DNAse II from Creative Enzymes has been used in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart. Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.
Form
essentially salt-free, lyophilized powder.
Enzyme Commission Number
EC 3.1.22.1
Activity
> 1,000 units/mg protein
Unit Definition
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM
Pathway
Clathrin derived vesicle budding, organism-specific biosystem; Lysosome, conserved biosystem; Membrane Trafficking, organism-specific biosystem
Function
deoxyribonuclease II activity
Synonyms
DNASE2; deoxyribonuclease II; EC 3.1.22.1; 9025-64-3; DNase II; pancreatic DNase II; deoxyribonucleate 3'-nucleotidohydrolase; DNase II; pancreatic DNase II; acid deoxyribonuclease; acid Dnase
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