Description
Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.
Abbr
GO, Recombinant (Bacillus subtilis)
Species
Bacillus subtilis
Enzyme Commission Number
EC 1.4.3.19
Molecular Weight
43.1 kDa (1-369 aa, NT His Tag)
Unit Definition
One unit is defined as the amount of enzyme required to convert one micromole of glycine into glyoxylate and hydrogen peroxide at pH 8.5 at 37°C.
Storage
Store at –20°C. Stable for at least 1 year as supplied. Avoid repeated freeze and thaw cycles.
Synonyms
Glycine oxidase; glycine oxygen oxidoreductase (deaminating); GO; EC 1.4.3.19; 39307-16-9
Download Datasheet