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Glycine Oxidase H244K from Bacillus subtilis, recombinant
Cat No.
NATE-1674
Description
Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.
Abbr
GO, Recombinant (Bacillus subtilis)
Source
E. coli
Species
Bacillus subtilis
Form
Liquid
Enzyme Commission Number
EC 1.4.3.19
Activity
1200 mU/mg
CAS No.
39307-16-9
Molecular Weight
43.1 kDa (1-369 aa, NT His Tag)
Purity
> 90% by SDS-PAGE
Concentration
4.0 mg/ml
Unit Definition
One unit is defined as the amount of enzyme required to convert one micromole of glycine into glyoxylate and hydrogen peroxide at pH 8.5 at 37°C.
Storage
Store at –20°C. Stable for at least 1 year as supplied. Avoid repeated freeze and thaw cycles.
Synonyms
Glycine oxidase; glycine oxygen oxidoreductase (deaminating); GO; EC 1.4.3.19; 39307-16-9
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