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DNA ligase (ATP or NAD+)

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Cat No.

EXWM-5825

Description

The enzymes from the archaea Thermococcus fumicolans and Thermococcus onnurineus show high activity with either ATP or NAD+, and significantly lower activity with TTP, GTP, and CTP. Different from EC 6.5.1.1, DNA ligase (ATP), EC 6.5.1.2, DNA ligase (NAD+) and EC 6.5.1.7, DNA ligase (ATP, ADP or GTP).

Form

Liquid or lyophilized powder

Enzyme Commission Number

EC 6.5.1.6

Storage

Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.

Reaction

(1) ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + diphosphate + (deoxyribonucleotide)n+m; (2) NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + β-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m

Notes

This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.