Description
Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule.Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
[BtrI acyl-carrier protein]-L-glutamate ligase; BtrJ
Reaction
(1) ATP + L-glutamate + BtrI acyl-carrier protein = ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]; (2) ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein] = ADP + phosphate + 4-(γ-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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