Description
Carboxypeptidase Y (CPY) catalyzes the following reaction: Peptidyl-L-amino acid + H2O ------> Peptide + L-amino acid.
It is prepared according to the method of Moore & Stein (J. Biol Chem, 211, 907, 1954). It resembles Carboxypeptidase A in its substrate specificity, but it hydrolyzes C-terminal glycine and L-leuicine more rapidly and L-phenylalanine more slowly.
Appearance
Clear, colorless to lightly colored
Form
500 mM sodium chloride, 500 mM imidazole, 20 mM sodium phosphate monobasic, 20 mM sodium phosphate dibasic, pH 7.5
Enzyme Commission Number
EC 3.4.16.1
Concentration
about 0.1 mg/mL
Unit Definition
One unit of enzyme activity is defined as that amount of enzyme that catalyzes the hydrolysis of 1 micromole of substrate per minute.
Storage
Long term below -20°C, short term 2-8°C. Avoid multiple freeze-thaws.
Synonyms
carboxypeptidase Y; serine carboxypeptidase I; cathepsin A; lysosomal protective protein; deamidase; lysosomal carboxypeptidase A; phaseolin; EC 3.4.16.5; 9046-67-7; Peptidyl-L-amino acid Hydrolase; Serine Carboxypeptidase; Carboxypeptidase C; Peptidyl-L-amino-acid (-L-proline ) hydrolase; EC 3.4.12.8
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