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Native Yeast Malate Dehydrogenase
Cat No.
NATE-1030
Description
Malate dehydrogenase is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is not to be confused with malic enzyme, which catalyzes the conversion of malate to pyruvate producing NADPH. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by pyruvate carboxylase to form oxaloacetate, a citric acid cycle intermediate. In order to get the oxaloacetate out of the mitochondria, malate dehydrogenase reduces it to malate, and it then traverses the inner mitochondrial membrane. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic malate dehydrogenase. Finally, phosphoenol-pyruvate carboxy kinase (PEPCK) converts oxaloacetate to phosphoenol pyruvate.
Abbr
MDH, Native (Yeast)
Alias
MDH
Source
Yeast
Form
Ammonium sulfate suspension
Enzyme Commission Number
EC 1.1.1.37
Activity
> 1,000 units/mg protein (at 25°C and pH 7.5)
CAS No.
9001-64-3
Contaminants
Fumarase (L-malate) < 0.01 % L-Lactate dehydrogenase (NADH) < 0.01 % Glutamic-oxalacetic transaminase < 0.01 % Glutamate dehydrogenase (NAD+) < 0.001 % NADH oxidase < 0.001%
Storage
1 -10°C
Synonyms
malic dehydrogenase; L-malate dehydrogenase; NAD-L-malate dehydrogenase; malic acid dehydrogenase; NAD-dependent malic dehydrogenase; NAD-malate dehydrogenase; NAD-malic dehydrogenase; malate NAD dehydrogenase; NAD-dependent malate dehydrogenase; NAD-sp; ECific malate dehydrogenase; NAD-linked malate dehydrogenase; MDH; L-malate-NAD+ oxidoreductase; S-malate: NAD+ oxidoreductase; EC 1.1.1.37; Malate Dehydrogenase
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1107 | L-Malate dehydrogenase from E. coli, Recombinant | EC 1.1.1.37 | 9001-64-3 | E. coli | Inquiry |
| NATE-0447 | Native Porcine Malic Dehydrogenase | EC 1.1.1.37 | 9001-64-3 | Porcine heart | Inquiry |
| DIA-278 | Native Porcine Malate Dehydrogenase, IFCC Quality | Porcine heart | Inquiry | ||
| NATE-0448 | Native Thermus flavus Malic Dehydrogenase | EC 1.1.1.37 | 9001-64-3 | Thermus flavus | Inquiry |