Description
Beta-porphyranase (EC 3.2.1.178, porphyranase, PorA, PorB, endo-beta-porphyranase) is an enzyme with systematic name porphyran beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate 4-glycanohydrolase. This enzyme catalyses the following chemical reaction: Hydrolysis of beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate linkages in porphyran. The backbone of porphyran consists largely (~70%) of (1->3)-linked beta-D-galactopyranose followed by (1->4)-linked alpha-L-galactopyranose-6-sulfate.
Abbr
β-Porphyranase, Recombinant (Zobellia galactanivorans)
Species
Zobellia galactanivorans
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.178
Molecular Weight
33.3 kDa
Purity
>90% as judged by SDS-PAGE
Optimum temperature
30 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
Beta-porphyranase; EC 3.2.1.178; porphyranase; PorA; PorB; endo-beta-porphyranase
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