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Native Pseudomonas sp. Isoamylase

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Cat No.

NATE-0360

Description

Isoamylase is an enzyme with system name glycogen 6-alpha-D-glucanohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins. This enzyme also readily hydrolyses amylopectin.

Abbr

Isoamylase, Native (Pseudomonas sp.)

Source

Pseudomonas sp.

Form

ammonium sulfate suspension; Supplied as a suspension in 3.2 M ammonium sulfate

Enzyme Commission Number

EC 3.2.1.68

Activity

180 U/mg protein (on oyster glycogen) at pH 4.0 and 40°C. 200 U/mL

CAS No.

9067-73-6

Purity

Single major band on SDS-gel electrophoresis (MW = 71,500).

Unit Definition

One Unit of isoamylase activity is defined as the amount of enzyme required to release one μmole of reducing sugar per minute from oyster glycogen (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40°C. One Unit as defined here is approximately equal to 67 KU as defined by Sigma for Isoamylase (rice starch as substrate at pH 3.5 and 40°C).

Storage

2-8°C

Synonyms

EC 3.2.1.68; debranching enzyme; glycogen alpha-1,6-glucanohydrolase; isoamylase

"Isoamylase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1212	Isoamylase from Escherichia coli, Recombinant	EC 3.2.1.68	9067-73-6	Escherichia coli str. K-12 substr. W3110	Inquiry
SUG-006	Isoamylase (Food Grade)	EC 3.2.1.68	9067-73-6		Inquiry
EXWM-3932	isoamylase	EC 3.2.1.68	9067-73-6		Inquiry