Description
The reaction is observed only in the direction of nitrous oxide reduction. Contains the mixed-valent dinuclear CuA species at the electron entry site of the enzyme, and the tetranuclear Cu-Z centre in the active site.In Paracoccus pantotrophus, the electron donor is cytochrome c552.
Species
Paracoccus denitrificans
Form
Liquid or Lyophilized powder
Enzyme Commission Number
EC 1.7.2.4
Purity
Greater than 90% as determined by SDS-PAGE.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reaction
nitrogen + H2O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H+
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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