Description
D-amino-acid dehydrogenase (EC 1.4.99.1) is a bacterial enzyme that catalyses the oxidation of D-amino acids into their corresponding oxoacids. It contains both flavin and nonheme iron as cofactors. The enzyme has a very broad specificity and can act on most D-amino acids. D-amino acid + H2O + acceptor <=> a 2-oxo acid + NH3 + reduced acceptor. This reaction is distinct from the oxidation reaction catalysed by D-amino acid oxidase that uses oxygen as a second substrate, as the dehydrogenase can use many different compounds as electron acceptors, with the physiological substrate being coenzyme Q. D-amino-acid dehydrogenase is used in enzyme assays to measure substrate specificity of D-amino acids, such as DauA.
Abbr
D-Amino acid dehydrogenase, Recombinant
Applications
D-amino-acid dehydrogenase is a bacterial enzyme that catalyses the oxidation of D-amino acids into their corresponding oxoacids. It contains flavin and nonheme iron as cofactors and has a broad specificity thereby acting on most D-amino acids.
Enzyme Commission Number
EC 1.4.99.1
Unit Definition
1 U corresponds to the amount of enzyme which reduces 1 μmol 2-ketoglutarate per minute at pH 9.0 and 25°C (cosubstrate NADPH).
Synonyms
D-Amino acid dehydrogenase; EC 1.4.99.1; 37205-44-0
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