For many biocatalytic processes, purified enzymes are the core of the machinery. Purification of enzymes is usually time-consuming and costly. Furthermore, enzymes usually require additional processing such as immobilization to improve the activity or stability. Different immobilization strategies need to be evaluated to determine the best solution.

The use of microorganisms, such as E.coli, as whole-cell biocatalysts is a convenient strategy to provide pre-immobilized enzymes. In this case, the enzyme acts in the natural cellular environment, which also protects the enzyme from destabilization and degradation. It avoids further laborious production and purification steps and allows repeated use in sequential reactions. To overcome restrictions such as accessibility of substrate and product and interference with other enzymes, surface-displayed enzymes are usually used in whole-cell biocatalyst systems.

Figure 1. Schematic representation of anchor proteins for surface display used in biocatalysis in Gram-negative (a-c) and Gram-positive (d-f) microorganisms.
(Applied Microbiology and Biotechnology, 2014)

Creative Enzymes offers professional services to eventually deliver whole-cell biocatalysts:

• Suitable or custom hosts for biocatalyst display.
• Design and development of display methods.
• Rational design for whole-cell biocatalysts and bioprocess.
• Biocatalytic efficiency quantification and optimization.
• Scaling-up of biocatalysts to industrial scales.

With decade-long experience on biocatalysis development and advanced technologies in molecular biology, Creative Enzymes would be happy to be involved in any step during development of whole-cell biocatalysts. Please contact us to get a complementary consultation session about your specific projects.

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Related Sections

• Enzyme Stabilization by Immobilization
• Industrial Enzyme Production
• Enzymology Assays